Widefield Fluorescence Microscope

Widefield microscopy for fluorescence and transmitted light applications. Phase contrast, DIC, and FRET, and fluorescent filter options are available. An incubation chamber with CO₂ with temperature controls is available for live cell imaging.

System Configuration

• Light source: Halogen lamp for bright-field microscopy, mercury lamp (X-Cite series 120 PC)
• Fluorescence Filter Cubes:  DAPI, GFP, CY3, CY5
• FRET Filters: CFP, YFP
• Objectives: 10x/0.3 Dry, 20x/0.8 Dry, 40X/0.6 Dry, 40x/1.2 Water, 63x/1.4 Oil, 63 X/1.4 oil (Ph3)
• Cameras: Axiocam 506 color, Axiocam 506 b&w, and Orca Flash 4.0 v3 CMOS
• Acquisition software: Zen Black (Zen 2.3)

Manufacturer: Zeiss

Equipment Model: Zeiss Axiovert 200M

Location: 121C, Carl R. Woese Institute for Genomic Biology

Contact Person: Austin Cyphersmith, 217-244-9654, ajcypher@igb.illinois.edu

FRET Information: https://www.microscopyu.com/applications/fret/basics-of-fret-microscopy

Köhler Illumination: https://www-app.igb.illinois.edu/core-docs/books/igb-core-training/page/kohler-illumination

Training Materials:

• Axiovision Take Off Guide
• Instructions for Advanced Techniques
• Quick Reference Guide(pdf version)