This is an image of a neuromuscular junction in a rat larynx, imaged with the LSM 710 confocal system and using two photon second harmonic generation imaging microscopy. The nerve terminal (red) attaches to the motor endplate (green) together with the muscle (grey SHG image) imaged with a GaAsP BiG detector without any label. This image shows how the neuromuscular junction interacts with the muscles of the vocal fold to cause contractions after receiving signals from the brain. By imaging the neuromuscular junctions with a variety of instruments including superresolution techniques, this project aimed to study the effects of age on neuromuscular junctions.