The goal of this project is to apply a single molecule resolution spatial transcriptomics method to the Honeybee brain. Here we have detected 3.3 million individual transcripts that represent 130 genes in a single Honeybee section. Each spot color represents a different gene. The brain section is approximately 4.5 x 3 µm in size. Sample was prepared by Prof. Hee-Sun Han's lab, and collected by Prof. Gene Robinson's lab. Images were taken using the Zeiss Axiovert 200M at the Carle R. Woese Institute for Genomic Biology's Core Facilities. Images were analyzed using Teton at Carl R. Woese Institute for Genomic Biology's Core Facilities.