“This is a SHG image of a FET colorectal cancer cell spheroid embedded in a 3D type-I collagen gel. The nuclei of the cancer cells at the center are stained with DAPI. This experiment shows how cancer spheroids reorganize collagen fibers around them through mechanical interaction with the fibers.”
The goal of this project is to apply a single molecule resolution spatial transcriptomics method to the Honeybee brain. Here we have detected 3.3 million individual transcripts that represent 130 genes in a single Honeybee section. Each spot color represents a different gene. The brain section is approximately 4.5 x 3 µm in size. Sample was prepared by Prof. Hee-Sun Han's lab, and collected by Prof. Gene Robinson's lab. Images were taken using the Zeiss Axiovert 200M at the Carle R. Woese Institute for Genomic Biology's Core Facilities. Images were analyzed using Teton at Carl R. Woese Institute for Genomic Biology's Core Facilities.
These images are transverse sections of stem-wood from two temperate deciduous tree species from Manistee, Michigan. The top image is from a White Oak (Quercus alba) tree while the bottom image is a Sugar Maple (Acer saccharum) tree. These sections were used in a study analyzing how ray parenchyma cells in wood are related to radial wood nutrient translocation within trees. The ray parenchyma can be identified as the long lines of symplastically connected cells that span across each section. Additionally, the Oak species on the top depicts a band of multiseriate rays that are multiple ray parenchyma wide. These cells are hypothesized to influence radial nutrient translocation within sapwood and are tied to many other plant functions such as the storage of non-structural carbohydrates (NSCs) and plant defense compounds.
The goal of the project is to understand the architecture of chromosomes during meiosis by:
1. Imaging the synaptonemal complex (Sycp) in the wild-type and mutant spermatocytes.
2. Imaging recombination nodule components.
The image on the left shows the localization of single fluorophores for the two homologous chromosome labeled with Sycp3-Flux640 and Sycp1-Flux680. The image on the right is the ratiometrically-separated fluorophores, which shows sycp1 (red) localized between the two homologs labeled with sycp3 (red). The microscope used is the Abberior Minflux and the image is generated using the imspector software.
The goal of this project is to understand the solute transport at the extracellular space of a rodent brain by intracranial injection. The tracer used is Texas red dextran 3 and it shows the convection flow path in the brain. The other label is the expression of the circadian gene (PER1) that has a VENUS reporter. This project is being funded by 1R61HL159948-01. The microscope used is the Miltenyi Ultramicroscope and image segmented with the IMARIS software.
This is a 3D reconstruction of Osmium-stained dissected head of a worker bee. The intact brain is highlighted in the center and resolved at 2 microns. The reconstructed volume was taken using the X-ray tomograph from the NSI X-5000 located at the Carl R. Woese Institute for Genomic Biology.
This is a 3D reconstruction of Osmium-stained dissected head of a worker bee. The intact brain is highlighted in the center and resolved at 2 microns. The reconstructed volume was taken using the X-ray tomograph from the NSI X-5000 located at the Carl R. Woese Institute for Genomic Biology.
Representative image of immunofluorescent staining of a hatched mouse blastocyst in control. DAPI-nuclear stain; phalloidin- F-actin; Cy3- inner cell mass (ICM); Cdx2- trophectoderm (TE). Image was taken using the Confocal-Zeiss LSM 880 airyscan microscope at the Carl R. Woese Institute for Genomic Biology's Core Facility.
Nature designed equine hooves to resist high impacts. This high-resolution micro-computed tomography image illustrates the tubular canals with intersecting bridges in three dimensions in an equine hoof wall. These intricate porous sub-structures, micrometers in cross-section, were imaged using an X5000 NSI High-Resolution Micro-CT and rendered in Imaris at the Carl R. Woese Institute at the Genomic Biology Core Facility.
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