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University of Illinois Urbana-Champaign

  • January 2023

    The image shows a 3D stack of SBF-SEM images of human pancreatic neuroendocrine cancer cells undergoing autophagy dependent cell death. Chloroquine inhibits autophagy by inhibiting the degradation of autolysosome. This 3D image clearly shows that accumulation of autophagic vacuoles (autophagosome and autolysosome) were detected in chloroquine treated BON (human pancreatic neuroendocrine cancer) cells. The image was taken using SBF-SEM at the Carle R. Woese Institute for Genomic Biology’s core facilities

    3D Reconstruction Of Human Pancreatic Neuroendocrine Cancer Cells Undergoing Autophagy Dependent Cell Death.

    The image shows a 3D stack of SBF-SEM images of human pancreatic neuroendocrine cancer cells undergoing autophagy dependent cell death. Chloroquine inhibits autophagy by inhibiting the degradation of autolysosome. This 3D image clearly shows that accumulation of autophagic vacuoles (autophagosome and autolysosome) were detected in chloroquine treated BON (human pancreatic neuroendocrine cancer) cells. The image was taken using SBF-SEM at the Carle R. Woese Institute for Genomic Biology’s core facilities and segmented with the Amira 3D software.

     

    Instrument: Zeiss Sigma VP SBF-SEM and Amira 3D Software