Atomic Force Microscopy reveals detailed nanoscale collagen fibril arrangement in pig tendon captured in a 4*4 μm scan. Fibril diameters range from 120 nm to 150 nm, with D-band periodicity approximately 67 nm. This precise measurement of fibril dimensions and their arrangement into fibers offers key insights into the structural pattern of collagenous tissues. Such understanding is crucial for pathological analysis, especially for looking at fibrotic conditions, where collagen deposition is a key factor. This work was done by Mahmuda Rakib Arshee from Amy Wagoner-Johnson lab. Image was taken with the Cypher AFM.