System: Imaging Cytometer (iCyte)

Caspase-3 DAB and Hematoxylin staining of tissue sections of mouse tumors. The iCyte allows you to quantify the signal intensity of Caspase-3 in tumor vs non tumor cells.

The iCyte is a laser scanning (automated and quantitative imaging) cytometer which combines digital microscopy with image processing and the real time population data analysis of analytical cytometry. It is a non-confocal system that utilizes laser-spot scanning illumination. The detection system, which combines photo PMTs for fluorescence detection and photodiodes (PD) for absorbance and scatter detection, offers excellent quantitative cytometric performance and imaging capabilities.

The instruments' unique design allows simultaneous use of fluorescent and chromatic dyes. There are numerous cellular applications of iCyte to areas of study including histochemistry, cell cycle/mitosis, DNA damage, apoptosis, cell-cell interactions, circulating tumor cells, siRNA, translocation/cell signaling etc.