Imaging 3D structure of pollen is important in identification of the pollen species. The LSM 880 Airyscan system provides around 140nm resolution with 405nm excitation. Using the superior sensitivity and resolution of the Airyscan detector,  the spikes, surface structure, entine and exine are clearly resolved through the entire depth of the pollen (~40 micron through the Z). The Sunflower (Helianthus sp.) pollen was labeled with Phloxine B and processed after collection (Helianthus sp.) from the Burlington, NC area.