Confocal - Zeiss LSM 700 Microscope

The Zeiss LSM 700 confocal microscope system uses a Zeiss AXIO Observer Z1 inverted microscope stand with transmitted (HAL) and laser illumination sources. It can collect transmitted light images (via a T-PMT) as well as conventional confocal fluorescence images. Modues for tiled images, z-stacks, and time-lapse imaging are available.

System Configuration:

• Light source: Halogen lamp for bright-field microscope, mercury lamp for direct visualization of fluorescent specimens through the eyepiece
• Fluorescence Filters for eyepiece viewing only: DAPI, FITC (green), and DsRed
• Laser Lines: 405 nm (DAPI), 488 nm (GFP/YFP/FITC/Alexa 488/Cy2), 555 nm (tdTomato/mCherry/TRITC/Cy3), 639 nm (Alexa 647/Cy5)
• Objectives:10x/0.3 Dry, 20x/0.8 Dry, 40x/1.3 Oil, 63x/1.4 Oil
• Detectors: 2 PMTs for fluorescence (PMT1, PMT2), 1 PMT for Differential Interference Contrast (DIC) (T-PMT)
• Acquisition software: Zen Black 2012

Information:

• Manufacturer: Zeiss
• Equipment Model: Zeiss LSM 700
• Location: 14B, Carl R. Woese Institute for Genomic Biology
• Contact Person: Kingsley Boateng, 217-300-1642, kboateng@illinois.edu
• LSM 700 Basic operation: https://www-app.igb.illinois.edu/core-docs/books/igb-core-training/page/lsm-700-basic-operation
• Zeiss LSM-700-Quick-guide: http://www.science.uwaterloo.ca/~spafford/davidspafford/Quick%20Guide%20LSM%207000_ZEN%202011ZSept%202011.pdf