Core Facilities Workshop | Carl R. Woese Institute for Genomic Biology

Office of the Vice Chancellor for Research and Innovation

Carl R. Woese Institute for Genomic Biology

2^nd Annual Midwest Advanced MINFLUX and Super Resolution Workshop

May 20-24, 2024

The IGB Core and Abberior Instruments are proud to host the 2nd annual Midwest MINFLUX and super resolution workshop May 20th to 24th 2024. The workshop is designed for both researchers who are new to MINFLUX and STED as well as those who would like to expand their knowledge. Stefan W. Hell will present the key note lecture on MINFLUX imaging. Hands on MINFLUX and STEDYCON sessions will take place in the afternoon and evenings.

If you are considering starting a MINFLUX or STEDYCON imaging project the IGB Core will work with you to prepare your sample. The Monday night reception will be preceded by short descriptions of the samples that will be imaged during the hands-on sessions.

Registration is now closed.

Workshop: Agenda

**All talks will be held in IGB 612 at 1206 West Gregory Dr. Urbana, IL 61801**

Monday, May 20
9:00-9:15 am CST	Welcome (Host)	Dr. Glenn Fried Director of the Core Facilities Carl R. Woese Institute for Genomic Biology University of Illinois Urbana Champaign
9:15-9:45 am CST	"STED microscopy - from Concepts to biological applications"	Dr. Christian Wurm Abberior Instruments America, Bethesda, USA CEO
9:45-10:15 am CST	“MINFLUX – The Concept”	Dr. Jessica Matthias Abberior Instruments America, Bethesda, USA Senior MINFLUX Applications Scientist
10:15-11:00 am CST	“3D imaging of photoreceptor synapses in tissue sections with DNA PAINT exchange”	Steven DeVries, MD, PhD Professor Ophthalmology & Neuroscience Northwestern University Feinberg School of Medicine
11:00-11:15 am CST	coffee break
11:15-12:00	"Processing...-Making the most out of MINFLUX data"	Dr. Otto Wirth Abberior Instruments America, Bethesda, USA MINFLUX Applications Scientist
12:00-1:00 pm CST	Lunch
1:00-3:00 pm CST	Live STED and MINFLUX demo (canned samples)
3:00-4:30 pm CST	Flash talks by workshop attendees – 5 minute intervals
4:30-6:00 pm CST	Reception - IGB Array Cafe Area
6:00-8:00 pm CST	measurement sessions

Tuesday, May 21
9:00-9:45 am CST	“Molecule-scale resolution and dynamics in fluorescence microscopy”	Prof. Dr. Dr. Stefan W. Hell Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany Max Planck Institute for Medical Research, Heidelberg, Germany
9:45-10:15 am CST	"Nanoscopy at synaptic structures"	Dr. Clara Guerth Abberior Instruments, Heidelberg, Germany MINFLUX Applications Scientist
10:15-10:45 am CST	"Super resolution microscopy to study the nanoscale organization of the cytoskeleton in neurons"	Dr. Victor Macarron Max Planck Institute for Medical Research, Heidelberg, Germany Postdoc
10:45-11:00am CST	Coffee Break
11:00-11:30 am CST	"MINFLUX Nanoscopy Reveals Ultra-fine Structure of Axial and Central Elements in Synaptonemal Complex"	Reza Rajabi-Toustani Postdoc Research Associate Carl R. Woese Institute for Genomic Biology University of Illinois Urbana-Champaign
11:30 am-12:00 pm CST	"Further Insights into Kinesin Motility Using MINFLUX: A Super-Resolution Nanoscopic Method"	Devinda Wijewardena Graduate Research Assistant Chemical & Biomolecular Engineering, Physics, National Science Foundation Science and Technology Center for Quantitative Cell Biology, University of Illinois Urbana-Champaign
12:00-12:30 pm CST	“MINFLUX for dynamic structural cell biology”	Prof. Dr. Jonas Ries Max Perutz Labs at the University of Vienna, Austria
12:30-1:30 pm CST	Lunch
1:30 – 3:30 pm CST	measurement sessions
3:40 – 5:40 pm CST	measurement sessions
5:50 – 7:50 pm CST	measurement sessions

Wednesday, May 22
9:00-9:45 am CST	“Exchangeable HaloTag Ligands for super-resolution fluorescence microscopy”	Julian Kompa Max Planck Institute for Medical Research, Heidelberg, Germany PhD student
9:45-10:30 am CST	"Applications of Caging Group Free Photoactivatable Fluorophores in STED and MINFLUX Nanoscopies"	Dr. Richard Lincoln Max Planck Institute for Medical Research, Heidelberg, Germany Postdoc
10:30-11:00 am CST	"Successful STED Imaging by Choosing the Optimal Probes - Dyes for Fixed and Living Samples"	Dr. Florian Grimm Abberior, Göttingen, Germany Business Developer
11:00-11:15 am CST	Coffee Break
11:15-11:45 am CST	"Sample Preparation and Labelling for STED Microscopy"	Dr. Mary Grace Velasco Abberior Instruments America, Bethesda, USA Senior STED Applications Scientist Dr. Olivier Brun Abberior Instruments America, Bethesda, USA Account Manager
11:45 am -12:15 pm CST	MINFLUX Applications & Sample Preparations	Dr. Kingsley Boateng Assistant Director of Bioscience Research and Microscopy Carl R. Woese Institute for Genomic Biology University of Illinois Urbana-Champaign
12:15-1:00 pm CST	Lunch
1:00-3:00 pm CST	measurement sessions
3:10-5:10 pm CST	measurement sessions
5:20-7:20 pm CST	measurement sessions

Thursday, May 23
9:00-11:00 am CST	measurement sessions
11:00 am - 1:00 pm CST	measurement sessions
1:00-2:00 pm CST	Lunch
2:00-4:00 pm CST	measurement sessions
4:10-6:10 pm CST	measurement sessions
6:20-8:20 pm CST	measurement sessions

Friday, May 24 - tentative
9:00-11:00 am CST	measurement sessions
11:10 am - 1:10 pm CST	measurement sessions
1:20-3:20 pm CST	measurement sessions

The university plans to capture photographs of this event. Be aware that if you attend, you are consenting to the possibility that your image and likeness may be shared or published by the university for marketing-communications purposes.

Workshop: Travel

Airports

Willard Airport, Champaign (CMI)

Central Illinois Regional Airport at Bloomington-Normal (BMI) 51 miles

O'Hare International Airport, Chicago (ORD) 154 miles

Indianapolis International Airport (IND) 116 miles

St. Louis Lambert International Airport (STL) 187 miles

Bus service from ORD, to Champaign

https://peoriacharter.com https://peoriacharter.com/storage/schedule/cu-01_2023.pdf

Urbana and University Meters

Mobile meter - https://www.parking.illinois.edu/meter-info/mobilemeter

Pay by Phone - https://www.paybyphone.com/?showDesktop=true

Parking information

https://www.parking.illinois.edu/sites/default/files/inline-files/parki… Extra meters can be found in F28.

Hotel

I Hotel - https://stayatthei.com/
(Runs shuttle to/from CMI, hotel and various campus buildings. Notify staff to use shuttle to IGB)

2023 Workshop Talks

IGB Core Facilities and Abberior Instruments co-hosted the first Midwest Advanced MINFLUX and Super Resolution Workshop from May 30th to June 2, 2023. Talks from the workshop are available for viewing.

view the 2023 talks

Workshop: Instructors

Dr. Stefan W. Hell

Director, Max Planck Institute (Gottingen And Heidelberg)

Max Planck Institute

Dr. Christian Wurm

CEO Abberior Instruments America

Abberior Instruments

Dr. Jessica Matthias

Senior MINFLUX Application Scientist

Abberior Instruments

Dr. Mary Grace Velasco

Senior STED Applications Scientist

Abberior Instruments

Dr. Kingsley Boateng

Assistant Director of Bioscience Research and Microscopy

Carl R. Woese Institute for Genomic Biology; University of Illinois Urbana-Champaign

Dr. Richard Lincoln

Postdoc, Synthetic Chemistry, Hell Lab Heidelberg

Max Planck Institute for Medical Research; Heidelberg, Germany

Steven DeVries, MD, PhD

Professor

Ophthalmology & Neuroscience; Northwestern University; Feinberg School of Medicine

Dr. Reza Rajabi-Toustani

Postdoc Research Associate

Department of Comparative Biosciences; College of Veterinary Medicine; University of Illinois Urbana-Champaign

Dr. Otto Wirth

MINFLUX Applications Scientist

Abberior Instruments America, Bethesda, USA

Dr. Clara Guerth

Application Specialist

Abberior Instruments

Dr. Victor Macarron

Max Planck Institute for Medical Research, Heidelberg, Germany

Devinda Wijewardena

Graduate Research Assistant

Chemical & Biomolecular Engineering, Physics; National Science Foundation Science and Technology Center for Quantitative Cell Biology; University of Illinois Urbana-Champaign