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Workshop

2nd Annual Midwest Advanced MINFLUX and Super Resolution Workshop

 

May 20-24, 2024

The IGB Core and Abberior Instruments are proud to host the 2nd annual Midwest MINFLUX and super resolution workshop May 20th to 24th 2024. The workshop is designed for both researchers who are new to MINFLUX and STED as well as those who would like to expand their knowledge. Stefan W. Hell will present the key note lecture on MINFLUX imaging. Hands on MINFLUX and STEDYCON sessions will take place in the afternoon and evenings.

If you are considering starting a MINFLUX or STEDYCON imaging project the IGB Core will work with you to prepare your sample. The Monday night reception will be preceded by short descriptions of the samples that will be imaged during the hands-on sessions.

 

Registration is now closed.

Workshop: Agenda

**All talks will be held in IGB 612 at 1206 West Gregory Dr. Urbana, IL 61801**

Monday, May 20

9:00-9:15 am CST Welcome (Host)

Dr. Glenn Fried
Director of the Core Facilities
Carl R. Woese Institute for Genomic Biology
University of Illinois Urbana Champaign

9:15-9:45 am CST "STED microscopy - from Concepts to biological applications"

Dr. Christian Wurm
Abberior Instruments America, Bethesda, USA
CEO

9:45-10:15 am CST “MINFLUX – The Concept”

Dr. Jessica Matthias
Abberior Instruments America, Bethesda, USA
Senior MINFLUX Applications Scientist

10:15-11:00 am CST “3D imaging of photoreceptor synapses in tissue sections with DNA PAINT exchange”

Steven DeVries, MD, PhD
Professor
Ophthalmology & Neuroscience
Northwestern University
Feinberg School of Medicine

11:00-11:15 am CST coffee break
11:15-12:00 "Processing...-Making the most out of MINFLUX data"

Dr. Otto Wirth
Abberior Instruments America, Bethesda, USA
MINFLUX Applications Scientist

12:00-1:00 pm CST Lunch
1:00-3:00 pm CST Live STED and MINFLUX demo (canned samples)
3:00-4:30 pm CST Flash talks by workshop attendees – 5 minute intervals
4:30-6:00 pm CST Reception - IGB Array Cafe Area
6:00-8:00 pm CST measurement sessions

 

Tuesday, May 21

9:00-9:45 am CST “Molecule-scale resolution and dynamics in fluorescence microscopy”

Prof. Dr. Dr. Stefan W. Hell
Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany
Max Planck Institute for Medical Research, Heidelberg, Germany

9:45-10:15 am CST "Nanoscopy at synaptic structures"

Dr. Clara Guerth
Abberior Instruments, Heidelberg, Germany
MINFLUX Applications Scientist

10:15-10:45 am CST "Super resolution microscopy to study the nanoscale organization of the cytoskeleton in neurons"

Dr. Victor Macarron
Max Planck Institute for Medical Research, Heidelberg, Germany
Postdoc

10:45-11:00am CST Coffee Break
11:00-11:30 am CST "MINFLUX Nanoscopy Reveals Ultra-fine Structure of Axial and Central Elements in Synaptonemal Complex"

Reza Rajabi-Toustani
Postdoc Research Associate
Carl R. Woese Institute for Genomic Biology
University of Illinois Urbana-Champaign

11:30 am-12:00 pm CST "Further Insights into Kinesin Motility Using MINFLUX: A Super-Resolution Nanoscopic Method"

Devinda Wijewardena
Graduate Research Assistant
Chemical & Biomolecular Engineering, Physics,
National Science Foundation Science and Technology Center for Quantitative Cell Biology,
University of Illinois Urbana-Champaign

12:00-12:30 pm CST “MINFLUX for dynamic structural cell biology”

Prof. Dr. Jonas Ries
Max Perutz Labs at the University of Vienna, Austria

12:30-1:30 pm CST Lunch
1:30 – 3:30 pm CST measurement sessions
3:40 – 5:40 pm CST measurement sessions
5:50 – 7:50 pm CST measurement sessions

 

Wednesday, May 22

9:00-9:45 am CST “Exchangeable HaloTag Ligands for super-resolution fluorescence microscopy”

Julian Kompa
Max Planck Institute for Medical Research, Heidelberg, Germany
PhD student

9:45-10:30 am CST "Applications of Caging Group Free Photoactivatable Fluorophores in STED and MINFLUX Nanoscopies"

Dr. Richard Lincoln
Max Planck Institute for Medical Research, Heidelberg, Germany
Postdoc

10:30-11:00 am CST "Successful STED Imaging by Choosing the Optimal Probes - Dyes for Fixed and Living Samples"

Dr. Florian Grimm
Abberior, Göttingen, Germany
Business Developer

11:00-11:15 am CST Coffee Break
11:15-11:45 am CST "Sample Preparation and Labelling for STED Microscopy"

Dr. Mary Grace Velasco
Abberior Instruments America, Bethesda, USA
Senior STED Applications Scientist

Dr. Olivier Brun
Abberior Instruments America, Bethesda, USA
Account Manager

11:45 am -12:15 pm CST MINFLUX Applications & Sample Preparations

Dr. Kingsley Boateng
Assistant Director of Bioscience Research and Microscopy
Carl R. Woese Institute for Genomic Biology
University of Illinois Urbana-Champaign

12:15-1:00 pm CST Lunch
1:00-3:00 pm CST measurement sessions
3:10-5:10 pm CST measurement sessions
5:20-7:20 pm CST measurement sessions

 

Thursday, May 23

9:00-11:00 am CST measurement sessions
11:00 am - 1:00 pm CST measurement sessions
1:00-2:00 pm CST Lunch
2:00-4:00 pm CST measurement sessions
4:10-6:10 pm CST measurement sessions
6:20-8:20 pm CST measurement sessions

 

Friday, May 24 - tentative

9:00-11:00 am CST measurement sessions
11:10 am - 1:10 pm CST measurement sessions
1:20-3:20 pm CST measurement sessions

The university plans to capture photographs of this event. Be aware that if you attend, you are consenting to the possibility that your image and likeness may be shared or published by the university for marketing-communications purposes.

 

 

Workshop: Travel

Airports

Willard Airport, Champaign (CMI)

Central Illinois Regional Airport at Bloomington-Normal (BMI) 51 miles

O'Hare International Airport, Chicago (ORD) 154 miles

Indianapolis International Airport (IND) 116 miles

St. Louis Lambert International Airport (STL) 187 miles

 

Bus service from ORD, to Champaign

https://peoriacharter.com https://peoriacharter.com/storage/schedule/cu-01_2023.pdf

 

Urbana and University Meters

Mobile meter - https://www.parking.illinois.edu/meter-info/mobilemeter

Pay by Phone - https://www.paybyphone.com/?showDesktop=true

 

Parking information

https://www.parking.illinois.edu/sites/default/files/inline-files/parki… Extra meters can be found in F28.

 

Hotel

I Hotel - https://stayatthei.com/
(Runs shuttle to/from CMI, hotel and various campus buildings. Notify staff to use shuttle to IGB)

2023 Workshop Talks

IGB Core Facilities and Abberior Instruments co-hosted the first Midwest Advanced MINFLUX and Super Resolution Workshop from May 30th to June 2, 2023. Talks from the workshop are available for viewing.

view the 2023 talks

Workshop: Instructors

Stefan W. Hell

Dr. Stefan W. Hell

Director, Max Planck Institute (Gottingen And Heidelberg)

Max Planck Institute

Dr. Christian Wurm

Dr. Christian Wurm

CEO Abberior Instruments America

Abberior Instruments

Dr. Jessica Matthias

Dr. Jessica Matthias

Senior MINFLUX Application Scientist

Abberior Instruments

Dr. Mary Grace Velasco

Dr. Mary Grace Velasco

Senior STED Applications Scientist

Abberior Instruments

Dr. Kingsley Boateng

Dr. Kingsley Boateng

Assistant Director of Bioscience Research and Microscopy

Carl R. Woese Institute for Genomic Biology; University of Illinois Urbana-Champaign

Dr. Richard Lincoln

Dr. Richard Lincoln

Postdoc, Synthetic Chemistry, Hell Lab Heidelberg

Max Planck Institute for Medical Research; Heidelberg, Germany

Steven DeVries, MD, PhD

Steven DeVries, MD, PhD

Professor

Ophthalmology & Neuroscience; Northwestern University; Feinberg School of Medicine

Dr. Reza Rajabi-Toustani

Dr. Reza Rajabi-Toustani

Postdoc Research Associate

Department of Comparative Biosciences; College of Veterinary Medicine; University of Illinois Urbana-Champaign

Dr. Otto Wirth

Dr. Otto Wirth

MINFLUX Applications Scientist

Abberior Instruments America, Bethesda, USA

Dr. Clara Guerth

Dr. Clara Guerth

Application Specialist

Abberior Instruments

Dr. Victor Macarron

Dr. Victor Macarron

Max Planck Institute for Medical Research, Heidelberg, Germany

Devinda Wijewardena

Devinda Wijewardena

Graduate Research Assistant

Chemical & Biomolecular Engineering, Physics; National Science Foundation Science and Technology Center for Quantitative Cell Biology; University of Illinois Urbana-Champaign

Prof. Dr. Jonas Ries

Prof. Dr. Jonas Ries

Max Perutz Labs
University of Vienna, Austria

Julian Kompa

Julian Kompa

Max Planck Institute for Medical Research; Heidelberg, German

Dr. Florian Grimm

Dr. Florian Grimm

Abberior, Göttingen, Germany

Dr. Olivier Brun

Dr. Olivier Brun

Abberior Instruments America, Bethesda, USA
Account Manager

Contacts

Glenn Fried Ph.D.

Glenn Fried PhD

Director of Core Facilities
gfried@igb.illinois.edu

Kingsley Boateng Ph.D.

Kingsley Boateng PhD

Assistant Director of Bioscience Research and Microscopy
kboateng@igb.illinois.edu

Hannah McClellan

Hannah McClellan

Administrative Support
hlm@illinois.edu

For Abberior specific questions

Christian Wurm

Dr. Christian Wurm

CEO
Abberior Instruments America

Abberior Instruments
c.wurm@abberior-instruments.com

Jessica Matthias

Dr. Jessica Matthias

Application Scientist

Abberior Instruments
j.matthias@abberior-instruments.com