Minflux (Minimal photon FLUX) is a super resolution method that combines aspects of single molecule localization microscopy (SMLM) and Stimulated Emissions Depletion (STED) microscopy that can achieve far higher spatial and temporal resolution, while requiring far fewer fluorescence photons. 

System Configuration:

MINFLUX imaging with localization precisions of 2 nm (2D/3D) in biological samples on large fields of view (10 x 15μm).

MINFLUX tracks molecular movements at frequencies up to 10kHz, resolving molecular positions ever 100 μs.

Due to the low number of fluorescence photons required for each localization, single molecules can be tracked over seconds (e.g 28000 localizations at nm precision).

A fully automated stabilization system based on laser-illuminated fiducial markers stabilizes the sample to < 1 nm in 3D.

Minflux excitation laser lines: 640 nm, 560nm

Confocal excitation laser lines: 405nm, 485nm, 560 nm, and 640 nm

Two-color radiometric Minflux imaging with 640 nm or 560 nm excitation

IX83 inverted microscope stand equipped with a 1.4 NA 100x oil objective

Recommended fluorescent probes for Minflux: Alexa 647 for single probe, Sulfo-Cyanin 5 (sCy5) and CF680 for two probes, Atto 647N for ultra-high-speed tracking

Information:

Manufacturer: Abberior Instruments

Location: 121H, Carl R. Woese Institute for Genomic Biology

Contact Person: Glenn Fried (gfried@igb.uiuc.edu)